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DNA TUNER – A COMPUTER PROGRAM FOR THE CONSTRUCTION OF POLYLINKER SEQUENCES OF MOLECULAR VECTORS

Authors: U.S. Latynski, L.N. Valentovich

DNA tuner - is a web application tool written using the Java programming language and is designed for the construction of a polylinker sequence of molecular vectors. The program application (the application of the program) gives a combination of the restriction sites chosen by user resulting in the minimum length sequence with (in sequence of minimum length with) overlapping of equal fragments obtained. The program combines the restriction sites chosen by user so that the sequence obtained is of minimal length due to the equal fragments overlapping. For user convenience, the final sequence is translated in three reading frames (+1, + 2, + 3), using standard genetic code table and the presence of restriction endonuclease recognition sites is detected.

Our tool DNA tuner is freely available at http://www.bio.bsu.by/molbiol/dna-tuner/.